Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659757.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 1 Kg
HEPES, Free Acid (N-(2-Hydroxyethyl) Piperazine-N'-(2-Ethanesulfonic Acid)) is a high-quality biochemical reagent widely used as a buffer in biological and chemical research. The 1kg size is ideal for large-scale research laboratories that require a stable, high-purity buffer for routine use. HEPES is particularly useful in maintaining consistent pH levels in cell culture, enzyme assays, and protein analysis, where precise control of pH is critical. As a zwitterionic buffer, it provides excellent pH stability in the physiological range without interfering with biological reactions, making it an essential tool in a variety of biochemical and molecular applications.
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 250G
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 250G
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659846.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 100 G
PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt] is a high-quality laboratory reagent supplied in a 100g bottle. This sodium salt form of PIPES is used to prepare buffers with precise pH control, particularly in biological and biochemical research. The sodium salt version is more soluble in water than the free acid form, making it ideal for high-concentration buffer solutions. It is widely used in protein purification, enzymatic assays, and molecular biology applications that require pH stability under varying conditions. The 100g pack size is suitable for small-scale experiments, and the reagent provides a reliable means of controlling pH in critical laboratory workflows.
eMolecules 58884-35-8 | 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | 58884-35-8 | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659807.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Free Acid is a biochemical reagent used for maintaining a stable pH environment in biological and chemical applications. This 250g package is ideal for preparing buffered solutions for use in cell culture, protein analysis, and other biochemical experiments. HEPES, or 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, is widely used because of its excellent buffering capacity in the pH range of 6.8 to 8.2. It is commonly used in cell biology to maintain physiological pH during experiments. This reagent is highly stable, non-toxic to cells, and is ideal for applications that require precise pH control over extended periods of time.
Sigma Aldrich Fine Chemicals Biosciences MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 1132-61-2 | MFCD00006183 | 2.5KG
MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 2.5KG
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660276.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg
HEPES, Free Acid is a powerful biochemical reagent provided in a 5kg size, ideal for large-scale applications in biological and biochemical research. It helps maintain a stable pH in the range of 6.8–8.2, which is critical for a variety of assays, including cell culture, protein purification, and enzyme assays. The reagent's excellent buffering capacity ensures that pH remains stable throughout experiments, preventing fluctuations that could affect results. Its low toxicity and high stability make it particularly useful in biological systems, offering consistent performance and reproducible results for laboratories involved in cell-based studies and biochemical analysis.
![Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-10000.0.JPG-250.jpg)
Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra suitable for500G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.