Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
TRIS Amino (Enzyme Grade) buffer is a high-purity solution designed specifically for enzyme-related applications in molecular biology and biochemistry. This 5kg package provides an ample supply of this reagent, which is widely used for preparing enzyme buffers in assays such as enzyme-linked immunosorbent assays (ELISA), protein assays, and PCR. The TRIS Amino buffer maintains stable pH conditions critical for optimal enzyme activity, and its enzyme-grade quality ensures minimal impurities that could interfere with biochemical reactions. This buffer is ideal for use in experiments where precise pH control is required for enzymatic reactions, as well as for preparing buffers for enzyme activity studies and protein purification.
Teknova 100X TE Buffer, pH 8.0. 4L, Sterile, DNase/RNase Tested, PCR Grade. 2 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 250 g
HEPES | Purity: ≥99.5% | Mol Wt: 238.30 | 7365-45-9 | MFCD00006158 | 250 g
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Sigma Aldrich Fine Chemicals Biosciences PIPES >=99% (titration) | 5625-37-6 | MFCD00006159 | 25G
PIPES >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 25G
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 100ml
HEPES buffer solution 1 M in H2O | Mol Wt: | 7365-45-9 | MFCD00006158 | 100ml
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Sigma Aldrich Fine Chemicals Biosciences HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
Sigma Aldrich Fine Chemicals Biosciences HEPES solution | 7365-45-9 | MFCD00006158 | 500ml
HEPES solution | Mol Wt: 238.306 | 7365-45-9 | MFCD00006158 | 500ml
Sigma Aldrich Fine Chemicals Biosciences Hepes|7365-45-9 | MFCD00006158 | 100g
Hepes | Purity: 99.5% (titration) | MW: 238.3 | 7365-45-9 | MFCD00006158 | 100g