Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences BES 99.0 titration1KG
BES (N N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES a sulfonic acid-containing cross-linking agent induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set crystals, 100 g
BioPerformance Certified, pH 8.0, average Mw 141.8, Synonym: Tris crystals. The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
ILEX LIFE SCIENCES LLC TRYPSIN 10X P10-022100 100ML
PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 2.50% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Hampton Research HR2-781/1.0 M BIS-TRIS pH 5.5 - 100 ml
HR2-781/1.0 M BIS-TRIS pH 5.5 - 100 ml. Please note items from this supplier are non-returnable.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, anhydrous | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid BioUltra, anhydrous | Purity: 99% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Teknova 10X PBS Solution. 1000mL, Sterile. Endotoxin Tested.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 250G
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 250G
Sigma Aldrich Fine Chemicals Biosciences Phosphate-Citrate Buffer tablet | 50TAB
Phosphate-Citrate Buffer tablet | 50TAB
LICOR PBS Blocking Buffer and PVDF Kit (Single buffer kit)
The non-mammalian Intercept Blocker in these kits is in PBS (phosphate-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low-background for infrared Western blot success. This kit contains Intercept (PBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m)
Supply Solutions Red Blood Cell Lysing Buffer Hybri-Max™, 100mL
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
General Laboratory Products Phosphate Buffered Water, 9mL 100/pk
Phosphate Buffered Water - 9ml, 16x125mm polycarbonate tube, 100 per case. For use as a diluent in microbial limit testing of food and water.
Teknova 1M Tris-HCl, pH 8.5 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.